Hematology u2013 manual differentials






















A “Differential Count” 1. Stained peripheral blood smear is examined carefully using 40 x to x objective – usually oil immersion lens 2. white blood cells are counted 3. Cells are classified by morphology→ Neutrophils. MODERATE COMPLEXITY HEMATOLOGY/COAGULATION SPECIALTY: Hematology TEST COMPLEXITY: Moderate Examples of moderate complexity tests: Complete blood counts (CBCs) Automated differential Manual white blood cell (WBC) differential with no identification of atypical cells Smears for granulocytes (eosinophils) Manual reticulocyte count. In this situation, a differential is usually performed by counting 50 cells. A notation on the report must be made that only 50 white cells were counted. Multiply each percentage x 2. When the WBC is very high (50,/ L), a cell diff may be .


The blood differential test is used to diagnose a variety of medical conditions. These may include infections, autoimmune diseases, anemia, inflammatory diseases, and leukemia and other types of cancer. It is a common test that is frequently used as part of a general physical exam. Routine Hemogram Reference Intervals These reference intervals were established using our Advia Hematology Analyzer. The asterixed values are obtained from the Advia. The remaining values are determined by bench methods, including the differential cell count (cells are counted in a peripheral blood smear), number of nucleated red blood cells/ WBC, percentage and absolute reticulocyte. Supervisor, Hematology/Hemostasis University of Iowa Hospitals and Clinics Disclosure • I have nothing to disclose. 2 Objectives • Describe the steps necessary to implement the automated IG parameter • Describe the benefits of the automated IG over the manual differential • Discuss the methods used to educate the.


Figure Zigzag method of performing differential. Example: platelets/10 fields = 12 platelets per field. 12 X 20, = , platelets. Manual Differential Counts. 1. These counts are done in the same area as WBC and platelet estimates with the red cells barely touching. 2. Lab manual – Ex (Hematology) Characteristics of the Formed Elements and Blood Abnormalities Fill in the blank with the term that fits the description. 1. The oxygen and carbon dioxide carrying cell - RBC 2. Help the body fight infections and foreign substances - WBC 3. Form a clot to help the body stop bleeding - Platelets 4. Appendix 1 - Diff Quick Maintenance. Audit reference: Equipment Section - Maintenance Log for Quick Stainer. Blood Film Preparation Procedure. Audit reference: Test and Control Articles Section. Differential Truth Table Guideline. Appendix A - Differential Truth Table Worksheet (Word) Appendix B - Differential Truth Table Worksheet Example.

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