Fluidics station 450 manual

These protocols provide detailed instructions for using the Fluidics Station and GeneChip Scanner to automate the washing, staining and scanning of GeneChip expression probe arrays. After completing the procedures described in this chapter, the scanned probe array image .dat file) is ready for analysis. Follow Fluidics station prompts to install array and the stain mixes (vial ). Array Fluidics Protocol E. coli Genome Mini_prok2v1_ E. coli Antisense Genome Array ProkGE-WS P. aeruginosa Genome Array requires modified FlexMidi_euk2v3_ See table in Prokaryotic Manual for modifications. Affymetrix GeneChip Fluidics Station Terms of loan/booking. Available for loan - internal and external. Fingerprint. Explore the research areas in which this equipment has been used. These labels are generated based on the related outputs. Together they form a unique fingerprint.

Affymetrix Fluidics Station Manual c. Affymetrix Fluidics Station Manual d. Hybridization Oven Manual e. Data Analysis Fundamentals f. GeneChip® Operating Software Manual g. Microarray Suite User's Guide, Version 2 GeneChip® 3' IVT Express Kit User Manual Assay Overview The GeneChip 3' IVT Express Kit aRNA amplification procedure is depicted in Figure GeneChip® Fluidics Station Affymetrix Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, , Affymetrix). Hybridization protocol: Following fragmentation, 1 ug of cRNA were hybridized for 16 hr at 48C on GeneChip miRNA Array. GeneChips were washed and stained in the Affymetri Fluidics.

Fluidics Station The Fluidics Station is the new generation of fluidics stations for processing GeneChip® arrays. The Fluidics Station incorporates advanced design improvements that provide improved ease-of-use and true walk away freedom to dramatically improve efficiency in your genetic analysis. Extensive testing and comparison to. Washing and Staining the Probe Array on Fluidics Station 38 This manual describes in detail two configurations of the WT Assay with key differences. chapter 1 | The Fluidics Station 13 How the Fluidics Station Works The Fluidics Station is used to hybridize, wash, and stain the GeneChip ® probe arrays (called arrays in this manual). The FS can independently process an array using a different fluidics protocol in each of four different modules.